p35C-epegRNA-v2(for plant)

LOCUS      p35C-epegRNA-v2(for plant)            4122 bp DNA     circular SYN 16-JUL-2026
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    2760-p35C-epegRNA-v2(for plant)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   GenScript USA Inc
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     http://www.informaxinc.com/
            ORIGDB|GenBank
            ORIGDB|GenBank
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..4122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        402..836
                     /label=35Sen
     promoter        837..1299
                     /label=CmYLCV
     promoter        1308..1395
                     /label=U6 (88-bp)
     tRNA            1403..1473
                     /label=tGly
     misc_feature    1492..1528
                     /label=tevopre
     misc_feature    1536..1603
                     /label=HDV
     terminator      1612..1882
                     /label=HSP18.2t
     primer_bind     complement(1901..1917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1925..1941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1949..1979)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1994..2015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2288..2902)
                     /direction=LEFT
                     /label=rep(pMB1)
                     /note="/vntifkey=33"
                     /note="/vntifkey=33"
     rep_origin      complement(2303..2891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3062..3922)
                     /codon_start=1
                     /label=bla(ApR)
                     /note="/vntifkey=4"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3923..4027)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt catggagtca aagattcaaa
      421 tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac
      481 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact
      541 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
      601 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
      661 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
      721 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
      781 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgattggc
      841 agacatactg tcccacaaat gaagatggaa tctgtaaaag aaaacgcgtg aaataatgcg
      901 tctgacaaag gttaggtcgg ctgcctttaa tcaataccaa agtggtccct accacgatgg
      961 aaaaactgtg cagtcggttt ggctttttct gacgaacaaa taagattcgt ggccgacagg
     1021 tgggggtcca ccatgtgaag gcatcttcag actccaataa tggagcaatg acgtaagggc
     1081 ttacgaaata agtaagggta gtttgggaaa tgtccactca cccgtcagtc tataaatact
     1141 tagcccctcc ctcattgtta agggagcaaa atctcagaga gatagtccta gagagagaaa
     1201 gagagcaagt agcctagaag tagtcaaggc ggcgaagtat tcaggcacgt ggccaggaag
     1261 aagaaaagcc aagacgacga aaacaggtaa gagctaagca tctagataag ttgaaaacaa
     1321 tcttcaaaag tcccacatcg cttagataag aaaacgaagc tgagtttata tacagctaga
     1381 gtcgaagtag tgattgaaca aagcaccagt ggtctagtgg tagaatagta ccctgccacg
     1441 gtacagaccc gggttcgatt cccggctggt gcatgagacc ttcgggtctc acgcggttct
     1501 atctagttac gcgttaaacc aactagaatt tttttggccg gcatggtccc agcctcctcg
     1561 ctggcgccgg ctgggcaaca tgcttcggca tggcgaatgg gacttttttt tgatatctcc
     1621 ggggctaatt gaatatgaag atgaagatga aatatttggt gtgtcaaata aaaagctggt
     1681 gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa
     1741 tattaaatga atgtaagatc tcattataat gaataaacaa atgtttctat aatccattgt
     1801 gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact
     1861 atggaatatg attaaagata agaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1921 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1981 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2041 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2701 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3181 ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa
     3241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3361 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3661 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4021 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4081 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//